Interaction of 125 I-Labeled Colicin E1 with Escherichia coli
نویسندگان
چکیده
منابع مشابه
Initial steps of colicin E1 import across the outer membrane of Escherichia coli.
Data suggest a two-receptor model for colicin E1 (ColE1) translocation across the outer membrane of Escherichia coli. ColE1 initially binds to the vitamin B(12) receptor BtuB and then translocates through the TolC channel-tunnel, presumably in a mostly unfolded state. Here, we studied the early events in the import of ColE1. Using in vivo approaches, we show that ColE1 is cleaved when added to ...
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Conditions permitting survival (colony formation) of E. coli after treatment with colicin K have been found. Survival required K+ and Mg2+ at concentrations high enough to replace the intracellular ions lost from colicintreated cells. Either glucose minimal medium or broth could support survival. Survival was still observed after colicin-promoted efflux of Rb+ and decline in ATP levels had occu...
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A variety of probiotic bacteria have been tested to control animal and foodborne pathogenic bacteria in livestock. The mechanism of inhibition of pathogenic bacteria for several of those probiotic microorganisms is mediated by the production of bacteriocins. Colicins are probably the group of bacteriocins that have been most thoroughly characterized. Colicins are antimicrobial proteins produced...
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Treatment of the cell envelope of a colicin I-sensitive strain of Escherichia coli with the nonionic detergent Triton X-100 solubilizes an envelope component which forms a complex with 1251-colicin Ia and Ib. A similar component could not be shown in a strain known to be lacking active colicin I receptors. On this basis, this component is assumed to be the colicin I receptor. The colicin I rece...
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With worldwide concern over the use of antibiotics in animal agriculture and their contribution to the spread of antibiotic resistance, alternatives to conventional antibiotics are needed. Previous research in our laboratories has shown that colicin E1 is effective against some Escherichia coli strains responsible for postweaning diarrhea (PWD) in vitro. In this study we examined the efficacy o...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1982
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.150.3.1383-1390.1982